If you require a suitable positive control, please check the datasheet, which will often have a suggested positive control. Always ensure the tissue or cell line you use is from a tested species. Not all the datasheets will have a suggested suitable control, and we recommend the following in these circumstances:
Check to see the validation images of the antibody. Any tissues, cells or lysates that have been used successfully by these experiments can be considered a suitable positive control.
Try looking at the Swiss-Prot or Omnigene database links on the datasheet. These databases will often have a list of tissues that the protein is expressed in. These can also be considered suitable positive controls.
Check the GeneCards entry for the protein. This will usually provide you with relative levels of expression in various tissues.
If you still have difficulty finding a suitable control, we recommend doing a quick literature search on PubMed to see which tissues and cells express the protein of interest.
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